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Image Search Results
Journal: Oncology Letters
Article Title: Inhibitory effects of human lactoferrin on U14 cervical carcinoma through upregulation of the immune response
doi: 10.3892/ol.2013.1776
Figure Lengend Snippet: Collected anticoagulated blood from each group was diluted to 1×10 6 cell/ml, labeled with anti-mouse monoclonal antibodies and then incubated for 30 min with rabbit anti-mouse FITC-IgG monoclonal antibodies to CD4 + and CD8 + . Stained cells were examined with an EPICS-XL FACSCalibur flow cytometer using Expo 32 ADC software. (A) Normal mice; (B) PBS-treated mice; (C) Ad-GFP-treated mice; (D) Ad-hLF-treated mice. (E) The percentages of CD4 + T cells in PBS-, Ad-GFP- and Ad-hLF-treated mice and normal mice were 12.39±2.53, 12.85±3.62, 27.38±2.12 and 32.25±1.87%, respectively. (F) The percentages of CD8 + T cells in PBS-, Ad-GFP- and Ad-hLF-treated mice and normal mice were 10.24±2.17, 9.25±3.95, 19.52±3.14 and 25.79±3.14%, respectively. Different letter superscripts between values indicate a significant difference (P<0.05) and same letter superscripts between values indicate no significant difference (P>0.05). PBS, phosphate-buffered saline; Ad-GFP, adenovirus carrying green fluorescent protein; Ad-hLF, adenovirus carrying human lactoferrin.
Article Snippet: To investigate the effect of Ad-hLF on peripheral blood T-lymphocyte subpopulations of tumor-bearing mice, the collected anticoagulated blood from the Ad-hLF-treated group was diluted to 1×10 6 cells/ml, labeled with anti-mouse monoclonal antibodies (4A Biotech Co., Ltd., Beijing, China) for 30 min and then incubated for 30 min at 4°C with rabbit anti-mouse FITC-IgG monoclonal antibodies to CD4 + and
Techniques: Labeling, Bioprocessing, Incubation, Staining, Flow Cytometry, Software, Saline